* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-4162 uni2-3::NIT1 mt-
$30.00
From Brian Piasecki, Silflow lab, University of Minnesota, June 2007
Phenotype: uniflagellate
This is Piasecki’s strain 31E3, obtained by insertional mutagenesis. Most cells in a culture of this strain are either uniflagellate or have no flagella.
Piasecki BP, LaVoie M, Tam LW, Lefebvre PA, Silflow CD (2008) The Uni2 phosphoprotein is a cell cycle regulated component of the basal body maturation pathway in Chlamydomonas reinhardtii. Mol Biol Cell 19:262-273
Piasecki BP, Silflow CD (2009) The UNI1 and UNI2 genes function in the transition of triplet to doublet microtubules between the centriole and cilium in Chlamydomonas. Mol Biol Cell 20:368-378