CC-4164 gam10 mt-

$30.00

From Charlene Forest, Brooklyn College, August 2007

Phenotype: does not mate when grown at high temperature

This is a temperature-sensitive, sex-limited mutant defective in gamete fusion. When incubated overnight in N-free medium at the restrictive temperature, gam10 mt- cells can agglutinate with mt+ gametes, signal, activate their mating structures and adhere via their mating structures with activated wt+ mating structures but they cannot fuse. The gam11 mutant has a similar phenotype, but was independently isolated.


Forest CL (1983) Specific contact between mating structure membranes observed in conditional fusion-defective Chlamydomonas mutants. Exp Cell Res 148:143-154

Forest CL (1987) Genetic control of plasma membrane adhesion and fusion in Chlamydomonas gametes. J Cell Sci 88:613-621

Forest CL, Ojakian GK (1989) Mating Structure Differences Demonstrated by Freeze-Fracture Analysis of Fusion-Defective Chlamydomonas Gametes. The Journal of Protozoology 36:548-556