* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-4181 bld10-1 arg7 mt-
$30.00
From Masafumi Hirono, University of Tokyo, December 2007
Phenotype: lacks flagella, does not mate; requires arginine
The BLD10 locus encodes a coiled-coil protein localized to the cartwheel structure at the proximal end of the basal body. Null mutants at this locus have no basal bodies or flagella. Alleles in which a truncated protein is made have abnormal basal bodies in which the 9-fold symmetry is altered.
Marshall WF (2007) Centriole assembly: the origin of nine-ness. Curr Biol 7, R1057-R1059
Hiraki M, Nakazawa Y, Kamiya R, Hirono M (2007) Bld10p constitutes the cartwheel-spoke tip and stabilizes the 9-fold symmetry of the centriole. Curr Biol 17:1778-1783