CC-4268 sac1::ARG7 mt+ [ars5-3]
$30.00
From Jeffrey Moseley, Arthur Grossman group, Carnegie Institution of Washington, January 2009
This appears to be the sac1(ars5-3) strain used by Moseley et al. 2009, which they describe as the product of a cross of “CC-2267 x ars5-1″ (described in Davies et al. 1996). There was never a CC-2267 in the public stock collection, and we suspect that CC-2677 (cw15 nit1-305, 5D) is meant instead.
The sac1 mutants as a group are deficient in response to sulfur limitation: they lack arylsulfatase activity, do not take up sulfate as well as wild type cells, and do not synthesize the periplasmic proteins that normally accumulate during sulfur-limited growth. This strain can grow on nitrate medium.
Davies JP, Yildiz FH, Grossman A (1996) Sac1, a putative regulator that is critical for survival of Chlamydomonas reinhardtii during sulfur deprivation. EMBO J 15:2150-2159
Moseley JL, Gonzalez-Ballester D, Pootakham W, Bailey S, Grossman AR (2009) Genetic interactions between regulators of Chlamydomonas phosphorus and sulfur deprivation responses. Genetics 181:889-905