CC-428 ac-u-e-1-24 mt+
$30.00
Hurley Shepherd in Boynton-Gillham laboratory, Duke University
Phenotype: requires acetate
Woessner et al. found this mutant to be deficient in chloroplast ATP synthase activity, but Bennoun (pers. comm.) had difficulties in scoring it, and Harris found wild type colonies were produced in all matrix crosses; thus this mutant was not included in the complementation/recombination matrix in Woessner et al. (1984). The genetic lesion has not been identified.
Chloroplast DNA appears normal on digestion with BamHI or Eco RI.
Shepherd HS, Boynton JE, Gillham NW (1979) Mutations in nine chloroplast loci of Chlamydomonas affecting different photosynthetic functions. Proc Natl Acad Sci USA 76:1353-1357
Woessner JP, Masson A, Harris EH, Bennoun P, Gillham NW, Boynton JE (1984) Molecular and genetic analysis of the chloroplast ATPase of chlamydomonas. Plant Mol Biol 3:177-190
Myers AM, Grant DM, Rabert DK, Harris EH, Boynton JE, Gillham NW (1982) Mutants of Chlamydomonas reinhardtii with physical alterations in their chloroplast DNA. Plasmid 7:133-151