* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.

CC-4283 fla3-1

Name: CC-4283 fla3-1
SKU: CC-4283
Availability: InStock

$30.00

From Mary Porter, U of MN, March 2009

This is progeny clone 1B from a backcross of the original fla3-1 strain (CC-1391) to 21gr. The backcrossed strain is temperature sensitive for flagellar assembly at 32 degrees. The FLA3 gene encodes the KAP (kinesin associated protein) subunit of the kinesin 2 complex, which also includes the motor subunits FLA10 and FLA8. The fla3 mutation is a single base pair change at nucleotide 6853 of the cDNA sequence (T to C) and results in the change of a conserved phenylalanine to a leucine at amino acid residue 453.

Mueller J, Perrone CA, Bower R, Cole DG, Porter ME (2005) The FLA3 KAP subunit is required for localization of kinesin-2 to the site of flagellar assembly and processive anterograde intraflagellar transport. Mol Biol Cell 16:1341-1354

Locus:
FLA3 [KAP]
Chromosome:
10

CC-4283 fla3-1

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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