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CC-43 ac20 mt-

Name: CC-43 ac20 mt-
SKU: CC-43
Availability: InStock

$30.00

Boynton-Gillham laboratory, Duke University

Phenotype: cold sensitive on minimal medium

Product 5.6.1 from a cross of wild type to the original ac20 cr1 double mutant (CC-154 mt-, a strain now discontinued.)

This strain was used by Boynton et al. (1972) in their study of mutants deficient in chloroplast ribosomes, and defines the ac20 mutation in terms of chloroplast ribosome content (deficient in both ribosomal subunits). Like other mutants deficient in chloroplast ribosomes, ac20 is easiest to score by slow growth on minimal medium at cold temperature (15-17 degrees). Considered purely as a genetic marker, ac20 is not the best choice for this region of linkage group I. However, CC-43 appears to be a stable strain with the correct ac20 phenotype.

Herrin and colleagues determined that ac20 overaccumulates an unspliced 23S pre-RNA in the chloroplast, and that the primary defect in the mutant is in ITS processing rather than splicing.

Togasaki RK, Levine RP (1970) Chloroplast structure and function in ac-20, a mutant strain of Chlamydomonas reinhardi. I. CO2 fixation and ribulose-1,5-diphosphate carboxylase synthesis. J Cell Biol 44:531-539

Levine RP, Paszewski A (1970) Chloroplast structure and function in ac-20, a mutant strain of Chlamydomonas reinhardi. II. Photosynthetic electron transport. J Cell Biol 44:540-546

Goodenough UW, Levine RP (1970) Chloroplast structure and function in ac-20, a mutant strain of Chlamydomonas reinhardi. 3. Chloroplast ribosomes and membrane organization. J Cell Biol 44:547-562

Boynton JE, Gillham NW, Chabot JF (1972) Chloroplast ribosome deficient mutants in the green alga Chlamydomonas reinhardi and the question of chloroplast ribosome function. J Cell Sci 10:267-305

Harris EH, Boynton JE, Gillham NW (1974) Chloroplast ribosome biogenesis in Chlamydomonas. Selection and characterization of mutants blocked in ribosome formation. J Cell Biol 63:160-179

Herrin DL, Chen YF, Schmidt GW (1990) RNA splicing in Chlamydomonas chloroplasts. Self-splicing of 23 S preRNA. J Biol Chem 265:21134-21140

Thompson AJ, Herrin DL (1991) In vitro self-splicing reactions of the chloroplast group I intron Cr. LSU from Chlamydomonas reinhardtii and in vivo manipulation via gene-replacement. Nucl Acids Res 19:6611-6618

Holloway SP, Herrin DL (1998)Processing of a composite large subunit rRNA. Studies with chlamydomonas mutants deficient in maturation of the 23s-like rrna. Plant Cell 10:1193-1206

Locus:
AC20
Chromosome:
1

CC-43 ac20 mt-

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

Any opinions, findings, and conclusions or recommendations expressed on this website are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.

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