CC-4301 asq2 mt+

$30.00

From Wallace Marshall, UCSF, July 2009

The mutant was made by insertional mutagenesis of strain CC-849 cw10, and then the initial isolate was outcrossed twice to CC-125.
The strain is in a mt+ background and carries the asq2 mutation, which is in the gene TBCCD1. The phenotype of asq2 is more or less identical to that of vfl1 and vfl3, but we found it through a different initial phenotype and that’s why it has a different name. We know it is not vfl1 or vfl3 but we don’t know for sure that it isn’t allelic to any of the other vfl mutants that have been described in mapping studies by Silflow. The mutation causes loss of phototaxis, dissociation of mother-daughter centriole pairs, random variation of flagellar and basal body number, and randomization of daughter centriole position on the cell surface (this last phenotype is what the “asq” refers to , it means that the position of flagella is “askew”). The ASQ2 gene is on linkage group IX. The mutation was made by insertional mutagenesis with the aphVII hygromycin resistance cassette. We have cloned the gene, and found it to encode TBCCD1, which was not picked up in the Chlamydomonas genome because of bad sequence reads in the vicinity but we found it by synteny with the corresponding region of the volvox genome. We have cloned the entire Chlamydomonas gene and deposited the sequence in genbank, accession number EU816954.

We described the initial genetic identification of the asq2 mutant in the following paper:

Feldman JL, Geimer S, Marshall WF (2007) The mother centriole plays an instructive role in defining cell geometry. PLoS Biol 5:149

The cloning of asq2 is described in the following paper:

Feldman JL, Marshall WF (2009) ASQ2 encodes a TBCC-like protein required for mother-daughter centriole linkage and mitotic spindle orientation. Curr Biol 19:1238-43