CC-4349 cw15 mt+ [Goodenough 330A]

$30.00

From Zi Teng Wang, Goodenough lab, Washington University in St Louis, June 2010

This is the Goodenough lab strain 330A, a subclone of Ball’s strain 330 (CC-4324) that does not require arginine. Wang sent the following notes: “Strain 330 was sent to St. Louis from the Ball lab 1.5 years ago and inadvertently plated on TAP upon arrival, whereupon a non-arginine-requiring line, presumably (but not demonstrated experimentally to be) clonal, grew up and was used in subsequent experiments. Dr Sabine Waffenschmidt (U. Cologne) received an independent shipment from the Ball lab, made the same plating error, and also recovered a prototroph; hence the mutant presumably (but not demonstrated experimentally) arose initially in the Ball lab. No crosses have been performed to ascertain whether prototrophy is due to reversion or suppression. ”

March 21, 2013 – Genomic sequencing in the Merchant/Pellegrini lab and RNA-seq in their and the Goodenough lab has documented that CC-4349 is mt- and therefore cannot be CC-4324, the original mt+ strain parental to sta6 (CC-4333 and CC-4348).