CC-4374 cep290-1::NIT1 agg1 mt+
$30.00
From Deborah Cochran, George Witman lab, University of Massachusetts Medical School, November 2010, their F2D2
This is a mutant in which most of the CEP290 gene has been deleted. The Y168 stain (cep290-1:: NIT1, nit1,agg1,mt+) was obtained by insertional mutagenesis of the g1 strain ( (nit1, agg1, mt+; see Pazour et al. 1995) with the NIT1 plasmid pGP505. The cep290-1 strain was obtained by backcrossing the original mutant Y168 to CC-124. A mutant progeny from this first cross was backcrossed again to g1, and a mutant progeny from the second cross was designated as cep290-1.
Mutant cells are mostly palmelloid and have very short/stumpy flagella once released from the mother cell wall by treatment with autolysin.
Lechtreck KF, Johnson EC, Sakai T, Cochran D, Ballif BA, Rush J, Pazour GJ, Ikebe M, Witman GB (2009) The Chlamydomonas reinhardtii BBSome is an IFT cargo required for export of specific signaling proteins from flagella. J Cell Biol 187:1117-1132