CC-4375 ift46-1::NIT1 mt+
$30.00
From Deborah Cochran, George Witman lab, University of Massachusetts Medical School, November 2010, their YH6
This is an insertional mutation in the IFT46 gene. The cells are mostly palmelloid and have short, paralyzed flagella lacking dynein arms and with central pair defects.
The T8a4-11 stain (ift46-1::NIT1, nit1, pf1, mt+) was obtained by insertional mutagenesis of the KK30A3 strain ((nit1, pf1, mt+) with the NIT1 plasmid pMN24 by K.Kozminski and J. Rosenbaum (Yale University, New Haven, CT). The ift46-1 strain was obtained by crossing the original mutant T8a4-11 to CC-124 to get rid of the pf1 mutation.
Hou Y, Qin H, Follit JA, Pazour GJ, Rosenbaum JL, Witman GB (2007) Functional analysis of an individual IFT protein: IFT46 is required for transport of outer dynein arms into flagella. J Cell Biol 176:653-665