CC-4423 dhc1b-3 mt-
$30.00
From Benjamin Engel and Wallace Marshall, UCSF, June 28, 2011
This is an A to T transversion at position 12313 of the DHC1b coding sequence that results in an Ile to Phe substitution at amino acid 4105. The mutation was UV-induced in the CC-125 background, and the original strain was back-crossed twice to eliminate additional mutations that might have resulted from the UV exposure.
This strain was generated in the course of tetrad analysis for mapping purposes. The DHC1b locus is very close to the mating type locus, with recombination between the two of only about 1%.
Engel BD, Ishikawa H, Wemmer KA, Geimer S, Wakabayashi K, Hirono M, Craige B, Pazour GJ, Witman GB, Kamiya R, Marshall WF (2012) The role of retrograde intraflagellar transport in flagellar assembly, maintenance, and function. J Cell Biol 199:151-167
Shih SM, Engel BD, Kocabas F, Bilyard T, Gennerich A, Marshall WF, Yildiz A (2013) Intraflagellar transport drives flagellar surface motility. ELife 2