CC-4557 oda16-1::pODA16-HA rescue
$30.00
From David Mitchell, SUNY Upstate Medical University, January 2013
oda16-1 (mt-) was crossed with arg2 (mt+) to give oda16 arg2 which was co-transformed with ARG plasmid pJD67 and the ODA16 gene modified to express a 3xHA epitope tag at the carboxy-terminus. The swimming speed and dynein assembly rescued to WT.
Ahmed NT, Mitchell DR (2005) ODA16p, a Chlamydomonas flagellar protein needed for dynein assembly. Mol Biol Cell 16:5004-5012
Ahmed NT, Gao C, Lucker BF, Cole DG, Mitchell DR (2008) ODA16 aids axonemal outer row dynein assembly through an interaction with the intraflagellar transport machinery. J Cell Biol 183:313-322
Ahmed NT, Mitchell DR (2005) ODA16p, a Chlamydomonas flagellar protein needed for dynein assembly. Mol Biol Cell 16:5004-5012
Ahmed NT, Gao C, Lucker BF, Cole DG, Mitchell DR (2008) ODA16 aids axonemal outer row dynein assembly through an interaction with the intraflagellar transport machinery. J Cell Biol 183:313-322