* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-4597 GAF5 mt+ [isolate #13]
$30.00
From Duanmu Deqiang, Lagarias lab, University of California Davis, 8/22/13
To create this isolate, bilin-binding cyanobacteriochrome (CBCR) reporter NpF2164g5 from the cyanobacterium Nostoc punctiforme was transformed into the chloroplast genome of 4A+ (CC-4051).
This transformant line is resistant to 500 μg/mL Spectinomycin. The purified NpF2164g5 was shown to possess a covalently bound PCB chromophore. This line (#13) has higher level of protein expression.
Duanmu D, Casero D, Dent RM, Gallaher S, Yang W, Rockwell NC, Martin SS, Pellegrini M, Niyogi KK, Merchant SS, Grossman AR, Lagarias JC (2013) Retrograde bilin signaling enables Chlamydomonas greening and phototrophic survival. Proc Natl Acad Sci U S A 110:3621-3626