CC-4616 oda13-1 mt- [T7-2.1]
$30.00
From George Witman, University of Massachusetts Medical School, 1/10/14
This mutant was generated by transforming ptx2-1 with plasmid pGP535 (containing the PTX2 gene) to rescue ptx2 but not the oda13 (i.e., DLC6) deletion.
The ptx2-1 strain was produced by transforming strain g1 (nit1, NIT2, agg1, mt+; derivative of crosses between CC-124 and nit1-305) with pMN24.
Pazour GJ, Witman GB (2000) Forward and reverse genetic analysis of microtubule motors in Chlamydomonas. Methods 22:285-298
DiBella LM, Gorbatyuk O, Sakato M, Wakabayashi K, Patel-King RS, Pazour GJ, Witman GB, King SM (2005) Differential light chain assembly influences outer arm dynein motor function. Mol Biol Cell 16:5661-5674
The reference for the original ptx2-1 strain is:
Pazour GJ, Sineshchekov OA, Witman GB (1995) Mutational analysis of the phototransduction pathway of Chlamydomonas reinhardtii. J Cell Biol 131:427-440