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CC-4625 R107-4B mt+ (revertant of 76-5EN)

Name: CC-4625 R107-4B mt+ (revertant of 76-5EN)
SKU: CC-4625
Availability: InStock

$30.00

From Robert J. Spreitzer, University of Nebraska, March 2014

Phenotype: requires acetate at 35 °C, temperature-conditional

This photosynthesis-competent revertant was selected from the Rubisco rbcL-mRNA deficient 76-5EN nuclear mutant (Hong and Spreitzer 1994, 1998). It can grow on minimal medium at 25 °C, but dies on minimal medium at 35 °C. R107-4B is a double mutant (76-5EN/S107-4B) that results from a nuclear-gene intergenic suppressor (S107-4B) of 76-5EN. When separated from 76-5EN, S107-4B has a temperature-conditional phenotype (Hong and Spreitzer 1998). This strain has been maintained with acetate medium in darkness since its isolation.

Hong S, Spreitzer RJ (1994) Nuclear mutation inhibits expression of the chloroplast gene that encodes the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Plant Physiol 106:673-678

Hong S, Spreitzer RJ (1998) Nuclear-gene mutations suppress a defect in the expression of the chloroplast-encoded large subunit of ribulosebisphosphate carboxylase/oxygenase. Plant Physiol 116:1387-1392

CC-4625 R107-4B mt+ (revertant of 76-5EN)

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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