CC-4631 S118-1E mt- (suppressor of 76-5EN)

$30.00

From Robert J. Spreitzer, University of Nebraska, March 2014

This strain was recovered from a cross between R118-1E mt+ and “wild-type” mt- (Hong and Spreitzer 1998). It has been maintained with acetate medium in darkness since its isolation. The R118-1E photosynthesis-competent revertant was selected from the Rubisco rbcL-mRNA deficient 76-5EN nuclear mutant (Hong and Spreitzer 1994, 1998). When R118-1E mt+ was crossed with “wild-type” mt-, tetrad analysis indicated that R118-1E resulted from a nuclear-gene intergenic-suppressor mutation (S118-1E). Thus, S118-1E suppresses 76-5EN by restoring Rubisco holoenzyme expression.

Hong S, Spreitzer RJ (1994) Nuclear mutation inhibits expression of the chloroplast gene that encodes the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Plant Physiol 106:673-678

Hong S, Spreitzer RJ (1998) Nuclear-gene mutations suppress a defect in the expression of the chloroplast-encoded large subunit of ribulosebisphosphate carboxylase/oxygenase. Plant Physiol 116:1387-1392