CC-4632 R118-5E mt+ (revertant of 76-5EN)
$30.00
From Robert J. Spreitzer, University of Nebraska, March 2014
Phenotype: requires acetate at 35 °C, temperature-conditional
This photosynthesis-competent revertant was selected from the Rubisco rbcL-mRNA deficient 76-5EN nuclear mutant (Hong and Spreitzer 1994, 1998). It can grow on minimal medium at 25 °C, but dies on minimal medium at 35 °C. R118-5E could not be crossed because it may have a number of metabolic defects. This strain has been maintained with acetate medium in darkness since its isolation.
Hong S, Spreitzer RJ (1994) Nuclear mutation inhibits expression of the chloroplast gene that encodes the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Plant Physiol 106:673-678
Hong S, Spreitzer RJ (1998) Nuclear-gene mutations suppress a defect in the expression of the chloroplast-encoded large subunit of ribulosebisphosphate carboxylase/oxygenase. Plant Physiol 116:1387-1392