CC-4636 PAADA mt+

$30.00

From Robert J. Spreitzer, University of Nebraska, March 2014

Phenotype: spectinomycin resistant

Lawrence Bogorad gave this strain to Bob Spreitzer in 1995. It is presumed to have the rbcL 5′ promoter sequence -390 to +97 and rbcL 3′ sequence fused to the aadA gene, and this construction is inserted into the chloroplast genome after the 3′ end of atpB (Salvador et al. 1993). The strain can grow photosynthetically, and is resistant to 250 µg/mL spectinomycin when grow on acetate medium in darkness (Hong 1996). This strain was used to investigate the effect of the 76-5EN nuclear mutation (Hong and Spreitzer 1994) on chloroplast gene expression (Hong 1996).

Hong S (1996) Nuclear mutations affect the catalysis, stability, and expression of chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. Ph. D. thesis, University of Nebraska

Hong S, Spreitzer RJ (1994) Nuclear mutation inhibits expression of the chloroplast gene that encodes the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Plant Physiol 106:673-678

Salvador ML, Klein U, Bogorad L (1993) 5' Sequences are important positive and negative determinants of the longevity of Chlamydomonas chloroplast gene transcripts. Proc Natl Acad Sci USA 90:1556-1560