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CC-4640 PAADA/76-5EN(11-4) mt-

Name: CC-4640 PAADA/76-5EN(11-4) mt-
SKU: CC-4640
Availability: InStock

$30.00

From Robert J. Spreitzer, University of Nebraska, March 2014

Phenotype: requires acetate, sensitive to light

This is progeny 4 from tetrad 11 from a cross between PAADA mt+ and 76-5EN mt- (Hong 1996). The strain can grow on acetate medium with 50 µg/mL spectinomycin in darkness, but dies with 250 µg/mL spectinomycin, indicating that 76-5EN partially blocks expression of aadA from the engineered rbcL promoter (Hong 1996). This strain requires acetate for growth because 76-5EN also blocks expression of the native rbcL gene (Hong and Spreitzer 1994). It has been maintained with acetate medium in darkness since it was isolated.

Hong S (1996) Nuclear mutations affect the catalysis, stability, and expression of chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. Ph. D. thesis, University of Nebraska

Hong S, Spreitzer RJ (1994) Nuclear mutation inhibits expression of the chloroplast gene that encodes the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Plant Physiol 106:673-678

CC-4640 PAADA/76-5EN(11-4) mt-

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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