CC-4644 72-7JJ mt+ (rbc-5-72-7JJ) non-pf2 progeny used for genetic analysis

$30.00

From Robert J. Spreitzer, University of Nebraska, March 2014

Phenotype: requires acetate at 35 °C, temperature-conditional

The 72-7JJ nuclear mutant was isolated by Spreitzer et al. (1992) by screening a collection of acetate-requiring mutants for lack of complementation with a known Rubisco rbcL mutant (e. g., Spreitzer and Ogren 1983; Spreitzer et al. 1988). 72-7JJ grows on minimal medium at 25 °C, but dies on minimal medium at 35 °C. The mutant was crossed with pf2 mt- to confirm mendelian inheritance, and this mt+ progeny clone was retained for further analysis. 72-7JJ has normal whole-chain electron transport and chlorophyll levels. The 72-7JJ mutation is not in the Rubisco rbcS genes, but it causes a decrease in Rubisco stability and alters Rubisco catalytic properties (Hong 1996). When 72-7JJ mt+ was crossed with the very similar 68-11AR mt- mutant (Gotor et al. 1994), the two genes appeared to be linked (PD=12, NPD=0, T=1), and the 68-11AR/72-7JJ double mutant had a non-temperature-conditional, acetate-requiring phenotype (Hong 1996). When 68-11AR/72-7JJ mt+ was crossed with “wild-type” mt- a similar result was observed (PD=21, NPD=0, T=2). Thus, 68-11AR and 72-7JJ are separated by 4 map units. However, some clones of 72-7JJ have been observed to become acetate-requiring at 25 °C after many years of culture. This strain has been maintained with acetate medium in darkness since its isolation.

Hong S (1996) Nuclear mutations affect the catalysis, stability, and expression of chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. Ph. D. thesis, University of Nebraska

Spreitzer RJ, Al-Abed SR, Huether MJ (1988) Temperature-sensitive, photosynthesis-deficient mutants of Chlamydomonas reinhardtii. Plant Physiol 86:773-777

Gotor C, Hong S, Spreitzer RJ (1994) Temperature-conditional nuclear mutation of Chlamydomonas reinhardtii decreases the CO2/O2 specificity of chloroplast ribulose-bisphosphate carboxylase/oxygenase. Planta 193:313-319

Spreitzer RJ, Ogren WL (1983) Rapid recovery of chloroplast mutations affecting ribulosebisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 80:6293-6297

Spreitzer RJ, Thow G, Zhu G, Chen Z, Gotor C, Zhang D, Hong S (1992) Chloroplast and nuclear mutations that affect Rubisco structure and function in Chlamydomonas reinhardtii. Murata N ed Research in Photosynthesis 3:593-600