CC-4651 prk1-E267Amber mt- (F-60)
$30.00
From Robert J. Spreitzer, University of Nebraska, March 2014
Phenotype: requires acetate
Mutant F-60 was originally isolated as a photosynthesis-deficient strain that lacked phosphoribulokinase activity and protein (Bennoun and Levine 1967; Moll and Levine 1970; Roesler et al. 1992). Spreitzer received this F-60 mt- strain from Bob Togasaki in the late 1970s. The strain has been maintained with acetate medium in darkness since then, and has been cloned several times. Bryan D. Smith in Spreitzer’s group cloned and completely sequenced the prk1 gene from wild-type 2137 mt+ (GenBank: AF228914.1). When the prk1 gene of F-60 mt- was sequenced, an E267Amber (GAG-TAG) nonsense mutation was discovered (Smith 2000).
Bennoun P, Levine RP (1967) Detecting mutants that have impaired photosynthesis by their increased level of fluorescence. Plant Physiol 42:1284-1287
Moll B, Levine R (1970) Characterization of a photosynthetic mutant strain of Chlamydomonas reinhardtii deficient in phosphoribulokinase activity. Plant Physiol 46:576-580
Smith BD (2000) Isolation and analysis of Chlamydomonas reinhardtii phosphoribulokinase mutants and revertants. Undergraduate Honors Thesis, Department of Biochemistry, University of Nebraska (available from the Chlamydomonas Resource Center)