CC-4653 prk1-R64C mt- (12-2B) non-pf2 progeny used for genetic analysis
$30.00
From Robert J. Spreitzer, University of Nebraska, March 2014
Phenotype: requires acetate, sensitive to light
This strain was recovered from a cross between the original 12-2B mt+ isolate (CC-1227) and pf2 mt-. It has been maintained on acetate medium in darkness since its isolation, and was used to screen for additional prk1 mutants by Bryan D. Smith in Spreitzer’s group (Smith 2000).. The 12-2B mutant was recovered as a light-sensitive, acetate-requiring mutant (Spreitzer and Mets 1981), and was subsequently found to lack phosphoribulokinase activity (Salvucci and Ogren 1985) due to a missense mutation (R64C; CGC-TGC) in the prk1 gene (Roesler et al. 1992).
Roesler K, Marcotte B, Ogren W (1992) Functional importance of arginine 64 in Chlamydomonas reinhardtii phosphoribulokinase. Plant Physiol 98:1285-1289
Salvucci M, Ogren W (1985) A Chlamydomonas reinhardtii mutant with catalytically and structurally altered ribulose-5-phosphate kinase. Planta 165:340-347
Smith BD (2000) Isolation and analysis of Chlamydomonas reinhardtii phosphoribulokinase mutants and revertants. Undergraduate Honors Thesis, Department of Biochemistry, University of Nebraska (available from the Chlamydomonas Resource Center)
Spreitzer RJ, Mets L (1981) Photosynthesis-deficient mutants of Chlamydomonas reinhardtii with associated light-sensitive phenotypes. Plant Physiol 67:565-569