CC-4656 prk1-Δ50 mt+ (69-2P, original isolate)
$30.00
From Robert J. Spreitzer, University of Nebraska, March 2014
Phenotype: requires acetate, sensitive to light
A collection of over 350 acetate-requiring mutants (Spreitzer et al. 1988) was screened for complementation (Spreitzer and Ogren 1983) with the prk1-R64C mt- strain (12-2B mt-) (Smith 2000). Mutant 69-2P failed to complement, and lacked phosphoribulokinase activity and protein. Bryan D. Smith in Spreitzer’s group sequenced prk1, and discovered that 69-2P results from a 50-bp deletion (nucleotides 1296 through 1345 relative to the first nucleotide encoding the start codon; GenBank: AF228914.1) (Smith 2000). This strain has been maintained with acetate medium in darkness since its isolation.
Spreitzer RJ, Al-Abed SR, Huether MJ (1988) Temperature-sensitive, photosynthesis-deficient mutants of Chlamydomonas reinhardtii. Plant Physiol 86:773-777
Spreitzer RJ, Ogren WL (1983) Rapid recovery of chloroplast mutations affecting ribulosebisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 80:6293-6297
Smith BD (2000) Isolation and analysis of Chlamydomonas reinhardtii phosphoribulokinase mutants and revertants. Undergraduate Honors Thesis, Department of Biochemistry, University of Nebraska (available from the Chlamydomonas Resource Center)
Spreitzer RJ, Al-Abed SR, Huether MJ (1988) Temperature-sensitive, photosynthesis-deficient mutants of Chlamydomonas reinhardtii. Plant Physiol 86:773-777
Spreitzer RJ, Ogren WL (1983) Rapid recovery of chloroplast mutations affecting ribulosebisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 80:6293-6297
Smith BD (2000) Isolation and analysis of Chlamydomonas reinhardtii phosphoribulokinase mutants and revertants. Undergraduate Honors Thesis, Department of Biochemistry, University of Nebraska (available from the Chlamydomonas Resource Center)