CC-4658 prk1-Δ50 cwd arg7-8 mt- (69-2P)
$30.00
From Robert J. Spreitzer, University of Nebraska, March 2014
Phenotype: requires acetate, sensitive to light, lacks the cell wall
This strain was recovered by Bryan D. Smith in Spreitzer’s group from a cross between prk1-Δ50 mt+ and cwd arg7-8 mt- (CC-3395). The cwd arg7-8 mt- strain (Gumpel et al. 1994) was obtained from Don Weeks in 1994. This prk1-Δ50 cwd arg7-8 mt- strain lacks phosphoribulokinase activity and protein due to a 50-bp deletion in prk1 (Smith 2000), and may serve as a host for transformation with engineered prk1 genes. This strain has been maintained with acetate medium in darkness since its isolation. For unknown reasons, it no longer requires Arg for growth.
Gumpel NJ, Rochaix JD, Purton S (1994) Studies on homologous recombination in the green alga Chlamydomonas reinhardtii. Curr Genet 26:438-442
Smith BD (2000) Isolation and analysis of Chlamydomonas reinhardtii phosphoribulokinase mutants and revertants. Undergraduate Honors Thesis, Department of Biochemistry, University of Nebraska (available from the Chlamydomonas Resource Center)