CC-4659 prk1-Δ50 cwd mt- (69-2P)
$30.00
From Robert J. Spreitzer, University of Nebraska, March 2014
Phenotype: requires acetate, sensitive to light, lacks the cell wall
Bryan D. Smith in Spreitzer’s group transformed prk1-Δ50 cwd arg7-8 mt- with pARG7.8 (Gumpel et al. 1994) to eliminate the Arg requirement. This prk1-Δ50 cwd mt- strain lacks phosphoribulokinase activity and protein due to a 50-bp deletion in prk1 (Smith 2000), and may serve as a host for transformation with engineered prk1 genes. It has been maintained with acetate medium in darkness since its isolation.
Gumpel NJ, Rochaix JD, Purton S (1994) Studies on homologous recombination in the green alga Chlamydomonas reinhardtii. Curr Genet 26:438-442
Smith BD (2000) Isolation and analysis of Chlamydomonas reinhardtii phosphoribulokinase mutants and revertants. Undergraduate Honors Thesis, Department of Biochemistry, University of Nebraska (available from the Chlamydomonas Resource Center)
Gumpel NJ, Rochaix JD, Purton S (1994) Studies on homologous recombination in the green alga Chlamydomonas reinhardtii. Curr Genet 26:438-442
Smith BD (2000) Isolation and analysis of Chlamydomonas reinhardtii phosphoribulokinase mutants and revertants. Undergraduate Honors Thesis, Department of Biochemistry, University of Nebraska (available from the Chlamydomonas Resource Center)