* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.

CC-4664 prk1-R64C/G326S mt+ (R203-16A revertant of 12-2B)

Name: CC-4664 prk1-R64C/G326S mt+ (R203-16A revertant of 12-2B)
SKU: CC-4664
Availability: InStock

$30.00

From Robert J. Spreitzer, University of Nebraska, March 2014

Following MMS mutagenesis of prk1-R64C mt+ (CGC-TGC), Bryan Smith in Spreitzer’s group selected photosynthesis-competent revertants at a frequency of 1 X 10E-8 cells (Smith 2000). Revertant R203-16A has reduced photosynthetic growth relative to wild type. It was found to result from intragenic suppression that converts phosphoribulokinase Gly-326 (GGC) to Ser (AGC). The mutant protein has not been analyzed. This strain has been maintained with acetate medium in darkness since its isolation.

Smith BD (2000) Isolation and analysis of Chlamydomonas reinhardtii phosphoribulokinase mutants and revertants. Undergraduate Honors Thesis, Department of Biochemistry, University of Nebraska (available from the Chlamydomonas Resource Center)

Locus:
AC214 [PRK1]
Chromosome:
12

CC-4664 prk1-R64C/G326S mt+ (R203-16A revertant of 12-2B)

Products

Support

Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

Any opinions, findings, and conclusions or recommendations expressed on this website are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.

Cart