* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-4689 cnk2-1
$30.00
From Laura Hilton, Quarmby lab, Simon Fraser University, April 2014
A spectinomycin resistant cassette, CrAad, was inserted within the first intron of the 5′ UTR (JGI v5.5 gene ID Cre12.g560350). The mutant has slightly longer flagella than wild-type due to a reduced rate of flagellar disassembly at steady-state length. It has been crossed to 137c four times to reduce background mutations.
Hilton LK, Gunawardane K, Kim JW, Schwarz MC, Quarmby LM (2013) The kinases LF4 and CNK2 control ciliary length by feedback regulation of assembly and disassembly rates. Curr Biol 18; 23:2208-14
Meslet-Cladière L, Vallon O (2012) A new method to identify flanking sequence tags in chlamydomonas using 3'-RACE. Plant Methods 26:21