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CC-4690 rbcS∆-T60-3 mt- (cloned)

Name: CC-4690 rbcS∆-T60-3 mt- (cloned)
SKU: CC-4690
Availability: InStock

$30.00

From Robert J. Spreitzer, University of Nebraska, June 2014

Phenotype: requires acetate, sensitive to light

This is an isolate of CC-4415 that has been cloned for better growth and transformation frequency. The T60-3 mutant was recovered by transformation of cwd arg7-8 mt- (CC-3395) with pARG7.8 (Gumpel et al. 1994), which caused the deletion of the Rubisco rbcS gene family (rbcS1 and rbcS2) (Khrebtukova and Spreitzer 1996). T60-3 lacks a cell wall and flagella, and cannot be crossed. This strain has been maintained with acetate medium in darkness since its isolation.

12/21/23 – This strain was found to be wild-type for the small subunit so is not a clonal isolate of T60-3.

Gumpel NJ, Rochaix JD, Purton S (1994) Studies on homologous recombination in the green alga Chlamydomonas reinhardtii. Curr Genet 26:438-442

Khrebtukova I, Spreitzer RJ (1996) Elimination of the Chlamydomonas gene family that encodes the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Proc Natl Acad Sci USA 93:13689-13693

CC-4690 rbcS∆-T60-3 mt- (cloned)

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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