CC-4691 rbcS∆-CAL005.01.13 mt+ (dim1)

$30.00

From Robert J. Spreitzer, University of Nebraska, June 2014

Phenotype: requires acetate, sensitive to light

This mutant (originally named dim1) was received from Rachel Dent and Kris Niyogi in 2003. It was recovered by transformation of wild-type 4A mt+ (CC-4051) with a ble gene, which caused deletion of the Rubisco rbcS gene family (rbcS1 and rbcS2) (Dent et al. 2005). It has a cell wall and flagella, and has been used in experiments that required transformation and genetic crosses (Spreitzer et al. 2005; Genkov et al. 2006). This strain has been maintained with acetate medium in darkness since 2003.

Dent RM, Haglund CM, Chin BL, Kobayashi MC, Niyogi KK (2005) Functional genomics of eukaryotic photosynthesis using insertional mutagenesis of Chlamydomonas reinhardtii. Plant Physiol 137:545-556

Genkov T, Du YC, Spreitzer RJ (2006) Small-subunit cysteine-65 substitutions can suppress or induce alterations in the large-subunit catalytic efficiency and holoenzyme thermal stability of ribulose-1,5-bisphosphate carboxylase/oxygenase. Arch Biochem Biophys 451:167-174

Spreitzer RJ, Peddi SR, Satagopan S (2005) Phylogenetic engineering at an interface between large and small subunits imparts land-plant kinetic properties to algal Rubisco. Proc Natl Acad Sci USA 102:17225-17230