CC-4693 rbcS∆-CAL005.01.13 pf2 mt- (dim1) (pf2 progeny used for genetic analysis)
$30.00
From Robert J. Spreitzer, University of Nebraska, June 2014
Phenotype: requires acetate, sensitive to light
This pf2 mt- progeny clone was recovered from a cross between rbcS∆-CAL005.01.13 mt+ (Dent et al. 2005) and pf2 mt-. The mutant lacks the Rubisco rbcS1 and rbcS2 small-subunit genes, and has been used in experiments that required transformation and genetic crosses (Spreitzer et al. 2005; Genkov et al. 2006). This strain has been maintained with acetate medium in darkness since its isolation.
Dent RM, Haglund CM, Chin BL, Kobayashi MC, Niyogi KK (2005) Functional genomics of eukaryotic photosynthesis using insertional mutagenesis of Chlamydomonas reinhardtii. Plant Physiol 137:545-556
Genkov T, Du YC, Spreitzer RJ (2006) Small-subunit cysteine-65 substitutions can suppress or induce alterations in the large-subunit catalytic efficiency and holoenzyme thermal stability of ribulose-1,5-bisphosphate carboxylase/oxygenase. Arch Biochem Biophys 451:167-174
Spreitzer RJ, Peddi SR, Satagopan S (2005) Phylogenetic engineering at an interface between large and small subunits imparts land-plant kinetic properties to algal Rubisco. Proc Natl Acad Sci USA 102:17225-17230