CC-4694 rbcS1-WT mt- (WT-SS1)
$30.00
From Robert J. Spreitzer, University of Nebraska, June 2014
This strain was recovered by nuclear transformation of rbcS∆-T60-3 mt- with the rbcS1 gene, which encodes small-subunit 1 of Rubisco (Khrebtukova and Spreitzer 1996). Because it lacks the rbcS2 gene, and expresses half the normal amount of Rubisco, it can serve as a “wild-type” strain for comparison with engineered rbcS1 genes (Genkov and Spreitzer 2009). This strain has been maintained with acetate medium in darkness since its isolation to guard against selection for secondary mutations that may increase Rubisco expression.
Genkov T, Spreitzer RJ (2009) Highly conserved small subunit residues influence Rubisco large subunit catalysis. J Biol Chem 284:30105-30112
Khrebtukova I, Spreitzer RJ (1996) Elimination of the Chlamydomonas gene family that encodes the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Proc Natl Acad Sci USA 93:13689-13693