CC-4695 rbcS2-WT mt- (WT-SS2)
$30.00
From Robert J. Spreitzer, University of Nebraska, June 2014
This strain was recovered by nuclear transformation of rbcS∆-T60-3 mt- with the rbcS2 gene, which encodes small-subunit 2 of Rubisco (Khrebtukova and Spreitzer 1996). Because it lacks the rbcS1 gene, and expresses half the normal amount of Rubisco, it can serve as a “wild-type” strain for comparison with engineered rbcS2 genes (Genkov et al. 2006). This strain has been maintained with acetate medium in darkness since its isolation to guard against selection for secondary mutations that may increase Rubisco expression.
Genkov T, Du YC, Spreitzer RJ (2006) Small-subunit cysteine-65 substitutions can suppress or induce alterations in the large-subunit catalytic efficiency and holoenzyme thermal stability of ribulose-1,5-bisphosphate carboxylase/oxygenase. Arch Biochem Biophys 451:167-174
Khrebtukova I, Spreitzer RJ (1996) Elimination of the Chlamydomonas gene family that encodes the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Proc Natl Acad Sci USA 93:13689-13693