CC-4696 rbcL∆-MX3312 mt+
$30.00
From Robert J. Spreitzer, University of Nebraska, June 2014
Phenotype: requires acetate, sensitive to light
This Rubisco rbcL knock-out mutant was created by Genhai Zhu by replacing chloroplast rbcL of wild-type 2137 mt+ (CC-3269) with aadA (Satagopan and Spreitzer 2004; Zhu et al. 2010). It has been cloned and maintained with acetate medium in darkness since its isolation.
Satagopan S, Spreitzer RJ (2004) Substitutions at the Asp-473 latch residue of Chlamydomonas ribulosebisphosphate carboxylase/oxygenase cause decreases in carboxylation efficiency and CO2/O2 specificity. J Biol Chem 279:14240-14244
Zhu G, Kurek I, Liu L (2010) Engineering photosynthetic enzymes involved in CO2–assimilation by gene shuffling. In CA Rebeiz, C Benning, H Bohnert, H Daniell, JK Hoober, HK Lichtenthaler, AR Portis, BC Tripathy, eds, The Chloroplast: Basics and Applications, Springer, pp 307-322