CC-47 arg1 mt-
$30.00
Boynton-Gillham laboratory, Duke University
Phenotype: requires arginine
From R.P. Levine, brought to Duke University by N.W. Gillham, 1968. The arg1 mutant was UV-induced by Ebersold, and has been shown to be deficient in acetylglutamyl phosphate reductase. It can grow on arginine, ornithine or citrulline.
The original arg1 strain tended to bleach when deprived of arginine. A test cross of this strain to wild type CC-125 in 1979 showed 2:2 segregation for light-green, slow-growing colonies.
This mutant becomes suppressed easily. A suppressor that was EMS-induced by Loppes was reported by Levine and Goodenough (1970) to map to linkage group I. The arg1 mutant could also be suppressed by the ac141 suppressor described by Cosbey et al. (1965).
CC-861 arg1 mt+ is cleaner than this strain and easier to score.
Hastings PJ, Levine EE, Cosbey E, Hudock MO, Gillham NW, Surzycki SJ, Loppes R, Levine RP (1965) The Linkage groups of Chlamydomonas reinhardi. Microb Genet Bull 23:20-21
Levine RP, Goodenough UW (1970) The genetics of photosynthesis and of the chloroplast in Chlamydomonas reinhardi. Annu Rev Genet 4:397-408