CC-4700 rbcL∆-25B1 mt+ (non-pf2 progeny used for genetic analysis)
$30.00
From Robert J. Spreitzer, University of Nebraska, June 2014
Phenotype: requires acetate, sensitive to light
This 25B1 mt+ progeny clone, which is green in the dark, was recovered from a cross between 25B1 mt+ (CC-2803) and pf2 mt-. The 25B1 Rubisco rbcL knock-out mutant was created by chloroplast transformation and insertional mutagenesis (Newman et al. 1991). This strain has been used as a host for engineering the Rubisco large subunit (Zhu and Spreitzer 1994, 1996; Larson et al. 1997). It has been maintained with acetate medium in darkness since its isolation.
Larson EM, O'Brien CM, Zhu G, Spreitzer RJ, Portis AR Jr (1997) Specificity for activase is changed by a Pro-89 to Arg substitution in the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. J Biol Chem 272:17033-17037
Newman SM, Gillham NW, Harris EH, Johnson AM, Boynton JE (1991) Targeted disruption of chloroplast genes in Chlamydomonas reinhardtii. Molec Gen Genet 230:65-74
Zhu G, Spreitzer RJ (1994) Directed mutagenesis of chloroplast ribulose-bisphosphate carboxylase/oxygenase: Substitutions at large subunit asparagine 123 and serine 379 decrease CO2/O2 specificity. J Biol Chem 269:3952-3956
Zhu G, Spreitzer RJ (1996) Directed mutagenesis of chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase: Loop-6 substitutions complement for structural stability but decrease catalytic efficiency. J Biol Chem 271:18494-18498