CC-4702 rbcL-P89R mt+
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
Directed mutagenesis and chloroplast transformation of rbcL-W66Amber mt+ (Spreitzer et al. 1985) were used to create a P89R substitution (CCA to CGT) in the Rubisco large subunit, which alters the species specificity of the interaction between Rubisco and Rubisco activase (Larson et al. 1997). This is the original mutant strain. It has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve interactions between activase and Rubisco.
Larson EM, O'Brien CM, Zhu G, Spreitzer RJ, Portis AR (1997) Specificity for activase is changed by a Pro-89 to Arg substitution in the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. J Biol Chem 272:17033-17037
Spreitzer RJ, Goldschmidt-Clermont M, Rahire M, Rochaix JD (1985) Nonsense mutations in the Chlamydomonas chloroplast gene that codes for the large subunit of ribulosebisphosphate carboxylase/oxygenase. Proc Natl Acad Sci USA 82:5460-5464