* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-4704 rbcL-P89R pf2 mt- (pf2 progeny used for genetic analysis)
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
This is a pf2 mt- progeny clone obtained from a cross between rbcL-P89R mt+ (Larson et al. 1997) and pf2 mt-. The P89R substitution in the Rubisco large subunit alters the species specificity of the interaction between Rubisco and Rubisco activase (Larson et al. 1997). This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve interactions between activase and Rubisco.
Larson EM, O'Brien CM, Zhu G, Spreitzer RJ, Portis AR (1997) Specificity for activase is changed by a Pro-89 to Arg substitution in the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. J Biol Chem 272:17033-17037