CC-4706 rbcL-D94K mt+
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
Directed mutagenesis and chloroplast transformation of rbcL-W66Amber mt+ (Spreitzer et al. 1985) were used to create a D94K substitution (GAC-AAA) in the Rubisco large subunit, which alters the species specificity of the interaction between Rubisco and Rubisco activase (Ott et al. 2000). This is the original mutant strain. It has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve interactions between activase and Rubisco.
Ott CM, Smith BD, Portis AR, Spreitzer RJ (2000) Activase region on chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase: Nonconservative substitution in the large subunit alters species specificity of protein interaction. J Biol Chem 275:26241-26244
Spreitzer RJ, Goldschmidt-Clermont M, Rahire M, Rochaix JD (1985) Nonsense mutations in the Chlamydomonas chloroplast gene that codes for the large subunit of ribulosebisphosphate carboxylase/oxygenase. Proc Natl Acad Sci USA 82:5460-5464