* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-4707 rbcL-K356Q mt+
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
Directed mutagenesis and chloroplast transformation of rbcL∆-25B1 mt+ (CC-4700) were used to create a K356Q substitution (AAA-CAA) in the Rubisco large subunit to investigate the interaction between Rubisco and Rubisco activase (Larson et al. 1997). This is the original mutant strain. It has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may alter the interactions between activase and Rubisco.
Larson EM, O'Brien CM, Zhu G, Spreitzer RJ, Portis AR (1997) Specificity for activase is changed by a Pro-89 to Arg substitution in the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. J Biol Chem 272:17033-17037