CC-4712 rbcL-C172S mt+
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
Directed mutagenesis and chloroplast transformation of rbcL-W66Amber mt+ (Spreitzer et al. 1985) were used to create a C172S substitution (TGT-TCT) in the Rubisco large subunit to investigate stress-induced degradation of Rubisco (Moreno and Spreitzer 1999). The x-ray crystal structure of the mutant protein has been determined (Garcia-Murria et al. 2008). This is the original mutant strain. It has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco stability.
Garcia-Murria MJ, Karkehabadi S, Marin-Navarro J, Satagopan S, Andersson I, Spreitzer RJ, Moreno J (2008) Structural and functional consequences of the replacement of proximal residues Cys-172 and Cys-192 in the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase from Chlamydomonas reinhardtii. Biochem J 411:241-247
Moreno J, Spreitzer RJ (1999) Cys-172 to Ser substitution in the chloroplast-encoded large subunit affects stability and stress-induced turnover of ribulosebisphosphate carboxylase/oxygenase. J Biol Chem 274:26789-26793
Spreitzer RJ, Goldschmidt-Clermont M, Rahire M, Rochaix JD (1985) Nonsense mutations in the Chlamydomonas chloroplast gene that codes for the large subunit of ribulosebisphosphate carboxylase/oxygenase. Proc Natl Acad Sci USA 82:5460-5464