CC-4727 rbcS1-I58W3 pf2 mt+
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
Phenotype: requires acetate at 35 °C, temperature-conditional
Using plasmid pI58W3 (Esquivel et al. 2013), which would replace Ile-58 with three Trp residues (ATC-TGGTGGTGG) in the Rubisco small subunit, Todor Genkov in Spreitzer’s group transformed rbcS∆-CAL005.01.13 pf2 mt+ (CC-4692) (Dent et al. 2005). In contrast to the I58W3 mutant described previously (Esquivel et al. 2013), this strain has a cell wall and can be used in genetic crosses. It has been maintained with acetate medium in darkness since its isolation.
Dent RM, Haglund CM, Chin BL, Kobayashi MC, Niyogi KK (2005) Functional genomics of eukaryotic photosynthesis using insertional mutagenesis of Chlamydomonas reinhardtii. Plant Physiol 137:545-556
Esquivel MG, Genkov T, Nogueira AS, Salvucci ME, Spreitzer RJ (2013) Substitutions at the opening of the Rubisco central solvent channel affect holoenzyme stability and CO2/O2 specificity but not activation by Rubisco activase. Photosynth Res 118:209-218