CC-4732 rbcS1-Y68A mt-
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
Directed mutagenesis and nuclear-gene transformation of rbcS∆-T60-3 mt- (Khrebtukova and Spreitzer 1996) were used to create a Y68A substitution (TAC-GCC) in the betaA-betaB loop of the Rubisco small subunit, which causes decreases in Rubisco carboxylation catalytic efficiency and holoenzyme stability (Spreitzer et al. 2001; Spreitzer 2003; Esquivel et al. 2006). Mutant Y68A has also been studied with respect to enhanced hydrogen production (Pinto et al. 2013). This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function or stability.
Esquivel MG, Pinto TS, Marin-Navarro J, Moreno J (2006) Substitution of tyrosine residues at the aromatic cluster around the betaA-betaB loop of Rubisco small subunit affects the structural stability of the enzyme and the in vivo degradation under stress conditions. Biochemistry 45, 5745-5753
Khrebtukova I, Spreitzer RJ (1996) Elimination of the Chlamydomonas gene family that encodes the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Proc Natl Acad Sci USA 93:13689-13693
Pinto TS, Malcata FX, Arrabaça JD, Silva JM, Spreitzer RJ, Esquivel MG (2013) Rubisco mutants of Chlamydomonas reinhardtii enhance photosynthetic hydrogen production. Appl Microbiol Biotechnol 97:5635-5643
Spreitzer RJ (2003) Role of the small subunit in ribulose-1,5-bisphosphate carboxylase/oxygenase. Arch Biochem Biophys 414:141-149
Spreitzer RJ, Esquivel MG, Du YC, McLaughlin PD (2001) Alanine-scanning mutagenesis of the small-subunit betaA-betaB loop of chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase: Substitution at Arg-71 affects thermal stability and CO2/O2 specificity. Biochemistry 40:5615-5621