CC-4736 rbcS1-Y72A mt-
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
Phenotype: requires acetate at 35 °C, temperature-conditional
Directed mutagenesis and nuclear-gene transformation of rbcS∆-T60-3 mt- (Khrebtukova and Spreitzer 1996) were used to create a Y72A substitution in the betaA-betaB loop of the Rubisco small subunit, which causes decreases in Rubisco carboxylase activity and holoenzyme stability (Esquivel et al. 2006). Mutant Y72A has also been studied with respect to enhanced hydrogen production (Pinto et al. 2013). This mutant can grow on minimal medium at 25 °C, but dies on minimal medium at 35 °C. The strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function or stability.
Esquivel MG, Pinto TS, Marin-Navarro J, Moreno J (2006) Substitution of tyrosine residues at the aromatic cluster around the betaA-betaB loop of Rubisco small subunit affects the structural stability of the enzyme and the in vivo degradation under stress conditions. Biochemistry 45:5745-5753
Khrebtukova I, Spreitzer RJ (1996) Elimination of the Chlamydomonas gene family that encodes the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Proc Natl Acad Sci USA 93:13689-13693
Pinto TS, Malcata FX, Arrabaça JD, Silva JM, Spreitzer RJ, Esquivel MG (2013) Rubisco mutants of Chlamydomonas reinhardtii enhance photosynthetic hydrogen production. Appl Microbiol Biotechnol 97:5635-5643