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CC-4744 rbcS1-F132Ocher mt- (F132UAA)

Name: CC-4744 rbcS1-F132Ocher mt- (F132UAA)
SKU: CC-4744
Availability: InStock

$30.00

From Robert J. Spreitzer, University of Nebraska, July 2014

Directed mutagenesis and nuclear-gene transformation of rbcS∆-T60-3 mt- (CC-4415) were used to change the codon for Phe-132 (TTC) in the Rubisco small subunit to an ocher nonsense codon (TAA), which causes the deletion of nine carboxyl-terminal residues. The mutant Rubisco has decreases in carboxylation catalytic efficiency and thermal stability. This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function or stability.

Esquivel MG, Anwaruzzaman M, Spreitzer RJ (2002) Deletion of nine carboxyl-terminal residues of the Rubisco small subunit decreases thermal stability but does not eliminate function. FEBS Lett 520:73-76

Locus:
RBCS1
Chromosome:
2

CC-4744 rbcS1-F132Ocher mt- (F132UAA)

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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