CC-4745 rbcS1-S16A mt-

$30.00

From Robert J. Spreitzer, University of Nebraska, July 2014

Directed mutagenesis and nuclear-gene transformation of rbcS∆-T60-3 mt- (CC-4415) were used to create an S16A substitution (TCC-GCC) in the Rubisco small subunit, which causes a decrease in Rubisco holoenzyme stability (Genkov and Spreitzer 2009). This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function or stability.


Genkov T, Spreitzer RJ (2009) Highly conserved small subunit residues influence Rubisco large subunit catalysis. J Biol Chem 284:30105-30112


  • Locus:
  • RBCS1
  • Chromosome:
  • 2