* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-4752 rbcS1-Y32R mt-
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
Using established methods of directed mutagenesis and nuclear-gene transformation of rbcS∆-T60-3 mt- (CC-4415) (Genkov and Spreitzer 2009), Todor Genkov in Spreitzer’s group created a Y32R substitution in the Rubisco small subunit, which causes a decrease in Rubisco holoenzyme stability (Genkov and Spreitzer, unpublished). This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function or stability.
Genkov T, Spreitzer RJ (2009) Highly conserved small subunit residues influence Rubisco large subunit catalysis. J Biol Chem 284:30105-30112