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CC-4755 rbcS1-W73A mt-

Name: CC-4755 rbcS1-W73A mt-
SKU: CC-4755
Availability: InStock

$30.00

From Robert J. Spreitzer, University of Nebraska, July 2014

Phenotype: requires acetate at 35 °C, temperature-conditional

Directed mutagenesis and nuclear-gene transformation of rbcS∆-T60-3 mt- (CC-4415) were used to create a W73A substitution (TGG-GCC) in the Rubisco small subunit, which causes a decrease in Rubisco carboxylation catalytic efficiency and holoenzyme stability (Genkov and Spreitzer 2009). This mutant can grow on minimal medium at 25 °C, but dies on minimal medium at 35 °C. The strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function or stability.

Genkov T, Spreitzer RJ (2009) Highly conserved small subunit residues influence Rubisco large subunit catalysis. J Biol Chem 284:30105-30112

Locus:
RBCS1
Chromosome:
2

CC-4755 rbcS1-W73A mt-

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

Any opinions, findings, and conclusions or recommendations expressed on this website are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.

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