CC-4764 rbcS1-SSAT pf2 mt+
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
Phenotype: altered CO2 assimilation
Using a plasmid that encodes the Arabidopsis-Rubisco small subunit (Genkov et al. 2010), Todor Genkov in Spreitzer’s group transformed rbcS∆-CAL005.01.13 pf2 mt+ (CC-4692). In contrast to the SSAT mutant described previously (Genkov et al. 2010), this strain has a cell wall and can be used in genetic crosses. The strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function or stability.
Genkov T, Meyer M, Griffiths H, Spreitzer RJ (2010) Functional hybrid Rubisco enzymes with plant small subunits and algal large subunits: Engineered rbcS cDNA for expression in Chlamydomonas. J Biol Chem 285:19833-19841