CC-478 bld2 mt+
$30.00
From Ursula Goodenough, Harvard University, March 1978
Phenotype: lacks flagella, does not mate
The bld or bald mutants were recovered as nonmating mutants in Goodenough’s laboratory, and were subsequently shown to lack flagella.
The bld2 mutant was characterized as defective in basal body formation, but having normal levels of centrin and a stable nucleus-basal body connector. Dutcher et al. showed that the defect is in the gene encoding epsilon tubulin. The bld2-1 allele has a premature stop codon in this gene.
Goodenough UW, St. Clair HS (1975) BALD-2: a mutation affecting the formation of doublet and triplet sets of microtubules in Chlamydomonas reinhardtii. J Cell Biol 66:480-491
Ehler LL, Holmes JA, Dutcher SK (1995) Loss of spatial control of the mitotic spindle apparatus in a Chlamydomonas reinhardtii mutant strain lacking basal bodies. Genetics 141:945-960
Preble AM, Giddings TH Jr, Dutcher SK (2001) Extragenic bypass suppressors of mutations in the essential gene BLD2 promote assembly of basal bodies with abnormal microtubules in Chlamydomonas reinhardtii. Genetics 157:163-181
Dutcher SK, Morrissette NS, Preble AM, Rackley C, Stanga J (2002) Epsilon-tubulin is an essential component of the centriole. Mol Biol Cell 13:3859-3869